Cruise activities include:
- Micro and mesozooplankton samples will be collected through vertical hauling with WP2 40µm net and oblique hauling with double Bongo 200µm net
- Monthly, an oblique hauling will be made using a Bongo 335µm net, in order to collect icthyoplankton samples.
- Hydrographic profiles (temperature, conductivity and depth) will be taken using a CTD in the sample stations and in three more stations.
- An oceanographic rosette with Niskin bottles will collect biogeochemical (TA, pH, oxygen, inorganic nutrients, dissolved organic matter..) and biological samples (abundance, biomass and diversity) of planktonic organisms.
Sample processing and analysis techniques carried out in the Oceanographic center include:
- Zooplanktonic diversity and biomass analysis through traditional microscopy and semiautomatic identification (Zooscan and Flowcam- Ecotaxa)
- Cuantification and classification of marine planktonic microorganisms, according to their different optic and morphological properties, using flow citometry.
- Marine microorganisms diversity: DNA extraction, amplification of gen 16S rRNA by PC, High-Throughput Sequencing and HTS.
- Biogeochemical analysis: inorganic nutrients (Autoanalyzer AA4) and dissolved organic matter (quantity and quality)